Document Type

Article

Publication Date

1-1989

Publication Title

Journal of Bacteriology

Department

Department of Biological Sciences

Abstract

Extracts of the crude bacteroid fraction of symbiotically grown Bradyrhizobium japonicum were much more active in oxidizing protoporphyrinogen to protoporphyrin than were extracts of cells grown under free-living conditions, especially when assayed in atmospheres containing only traces of oxygen. This correlates with the higher heme content of the microaerophilic nodules. Furthermore, the high level of oxidative activity in the crude bacteroid fraction was associated with an uncharacterized membrane fraction, probably of plant origin, that was separable from the bacteroids by Percoll gradient centrifugation.

DOI

10.1128/jb.171.1.573-576.1989

Original Citation

Jacobs NJ, Borotz SE, Guerinot ML. Protoporphyrinogen oxidation, a step in heme synthesis in soybean root nodules and free-living rhizobia. J Bacteriol. 1989;171(1):573-576. doi:10.1128/jb.171.1.573-576.1989

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