Document Type

Article

Publication Date

3-30-2010

Publication Title

Applied and Environmental Microbiology

Department

Thayer School of Engineering

Abstract

The enrichment from nature of novel microbial communities with high cellulolytic activity is useful in the identification of novel organisms and novel functions that enhance the fundamental understanding of microbial cellulose degradation. In this work we identify predominant organisms in three cellulolytic enrichment cultures with thermophilic compost as an inoculum. Community structure based on 16S rRNA gene clone libraries featured extensive representation of clostridia from cluster III, with one cluster sharing as little as 73% sequence similarity with the closest known relative. In all, 14 new GHF48 gene sequences were added to the known diversity of 35 genes from cultured species.

DOI

10.1128/AEM.02689-09

Included in

Microbiology Commons

COinS