ENGS 88 Honors Thesis (AB Students)

Degree Program


Year of Graduation


Faculty Advisor

Wei-Lih Lee and Margaret Ackerman

Document Type

Thesis (Senior Honors)

Publication Date

Spring 6-10-2020


Current evidence suggests that, in Saccharomyces cerevisiae, the Num1 protein binds and anchors cytoplasmic dynein to the cell cortex, where dynein generates a pulling force that aligns the mitotic spindle during mitosis; however, the exact location where Num1 anchors dynein remains unclear. In this study, I genetically engineered fusions of the dynein-anchoring CC domain of Num1 to a resident ER protein, Tcb3. Through functional microscopy-based assays, I found that the fusion protein Tcb3-CC rescues the mitotic spindle alignment defect in num1Δ yeast, indicating that the cortical ER can serve as an anchoring platform for dynein.

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Engineering Commons