ENGS 88 Honors Thesis (AB Students)
Engineering An ER-Targeted Dynein-Anchoring Fusion Protein to Mediate Spindle and Nuclear Positioning in Budding Yeast
Year of Graduation
Wei-Lih Lee and Margaret Ackerman
Thesis (Senior Honors)
Current evidence suggests that, in Saccharomyces cerevisiae, the Num1 protein binds and anchors cytoplasmic dynein to the cell cortex, where dynein generates a pulling force that aligns the mitotic spindle during mitosis; however, the exact location where Num1 anchors dynein remains unclear. In this study, I genetically engineered fusions of the dynein-anchoring CC domain of Num1 to a resident ER protein, Tcb3. Through functional microscopy-based assays, I found that the fusion protein Tcb3-CC rescues the mitotic spindle alignment defect in num1Δ yeast, indicating that the cortical ER can serve as an anchoring platform for dynein.
Dartmouth Digital Commons Citation
Wang, Dayle, "Engineering An ER-Targeted Dynein-Anchoring Fusion Protein to Mediate Spindle and Nuclear Positioning in Budding Yeast" (2020). ENGS 88 Honors Thesis (AB Students). 20.