Document Type
Article
Publication Date
3-4-2011
Publication Title
Journal of Bacteriology
Department
Geisel School of Medicine
Abstract
Staphylococcus aureus RN4220, a cloning intermediate, is sometimes used in virulence, resistance, and metabolic studies. Using whole-genome sequencing, we showed that RN4220 differs from NCTC8325 and contains a number of genetic polymorphisms that affect both virulence and general fitness, implying a need for caution in using this strain for such studies.
DOI
10.1128/JB.00027-11
Original Citation
Nair D, Memmi G, Hernandez D, Bard J, Beaume M, Gill S, Francois P, Cheung AL. Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain. J Bacteriol. 2011 May;193(9):2332-5. doi: 10.1128/JB.00027-11. Epub 2011 Mar 4. PMID: 21378186; PMCID: PMC3133102.
Dartmouth Digital Commons Citation
Nair, Dhanalakshmi; Memmi, Guido; Hernandez, David; Bard, Jonathan; Beaume, Marie; Gill, Steven; Francois, Patrice; and Cheung, Ambrose L., "Whole-Genome Sequencing of Staphylococcus aureus Strain RN4220, a Key Laboratory Strain Used in Virulence Research, Identifies Mutations That Affect Not Only Virulence Factors but Also the Fitness of the Strain" (2011). Dartmouth Scholarship. 1057.
https://digitalcommons.dartmouth.edu/facoa/1057