Although the contribution of alveolar type II epithelial cell (AEC II) activities in various aspects of respiratory immune regulation has become increasingly appreciated, our understanding of the contribution of AEC II transcriptosome in immunopathologic lung injury remains poorly understood. We have previously established a mouse model for chronic T cell-mediated pulmonary inflammation in which influenza hemagglutinin (HA) is expressed as a transgene in AEC II, in mice expressing a transgenic T cell receptor specific for a class II-restricted epitope of HA. Pulmonary inflammation in these mice occurs as a result of CD4+ T cell recognition of alveolar antigen. This model was utilized to assess the profile of inflammatory mediators expressed by alveolar epithelial target cells triggered by antigen-specific recognition in CD4+ T cell-mediated lung inflammation. We established a method that allows the flow cytometric negative selection and isolation of primary AEC II of high viability and purity. Genome wide transcriptional profiling was performed on mRNA isolated from AEC II isolated from healthy mice and from mice with acute and chronic CD4+ T cell-mediated pulmonary inflammation.
Gereke, Marcus; Gröbe, Lothar; Prettin, Silvia; Kasper, Michael; Deppenmeier, Stefanie; Gruber, Achim D.; Enelow, Richard I.; Buer, Jan; and Bruder, Dunja, "Phenotypic Alterations in Type II Alveolar Epithelial Cells in CD4+ T Cell Mediated Lung Inflammation" (2007). Open Dartmouth: Faculty Open Access Articles. 1143.