Document Type


Publication Date


Publication Title

Journal of Neuroinflammation


Geisel School of Medicine


While it is clear that inbred strains of mice have variations in immunological responsiveness, the influence of genetic background following tissue damage in the central nervous system is not fully understood. A cortical explant system was employed as a model for injury to determine whether the immediate transcriptional response to tissue resection revealed differences among three mouse strains. Immunological mRNAs were measured in cerebral cortex from SJL/J, C57BL/6J, and BALB/cJ mice using real time RT-PCR. Freshly isolated cortical tissue and cortical sections incubated in explant medium were examined. Levels of mRNA, normalized to β-actin, were compared using one way analysis of variance with pooled samples from each mouse strain.



Original Citation

Graber DJ, Harris BT, Hickey WF. Strain-dependent variation in the early transcriptional response to CNS injury using a cortical explant system. J Neuroinflammation. 2011 Sep 26;8:122. doi: 10.1186/1742-2094-8-122. PMID: 21942980; PMCID: PMC3192692.