Document Type

Article

Publication Date

8-1-2012

Publication Title

Biomedical Optics Express

Department

Geisel School of Medicine

Abstract

Diffuse optical remission spectra from the mammalian neocortex at visible wavelengths contain spectral features originating from the mitochondria. A new algorithm is presented, based on analytically relating the first differential of the attenuation spectrum to the first differential of the chromophore spectra, that can separate and calculate the oxidation state of cytochrome c as well as the absolute concentration and saturation of hemoglobin. The algorithm is validated in phantoms and then tested on the neocortex of the rat during an anoxic challenge. Implementation of the algorithm will provide detailed information of mitochondrial oxygenation and mitochondrial function in physiological studies of the mammalian brain.

DOI

10.1364/BOE.3.001933

Original Citation

Sakata Y, Abajian M, Ripple MO, Springett R. Measurement of the oxidation state of mitochondrial cytochrome c from the neocortex of the mammalian brain. Biomed Opt Express. 2012 Aug 1;3(8):1933-46. doi: 10.1364/BOE.3.001933. Epub 2012 Jul 25. PMID: 22876356; PMCID: PMC3409711.

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