Background: Inhibitor of differentiation 4 (Id4), a member of the helix-loop-helix family of transcriptional regulators has emerged as a tumor suppressor in prostate cancer. Id4 is expressed in the normal prostate where its expression is also regulated by androgens. In this study we investigated the effect of loss of Id4 (Id4-/-) on adult prostate morphology. Methods: Histological analysis was performed on prostates from 6-8 weeks old Id4-/-, Id4+/- and Id4+/+ mice. Expression of Id1, Sox9, Myc, androgen receptor, Akt, p-Akt, Pten and Nkx3.1 was investigated by immunohistochemistry. Androgen receptor binding on NKX3.1 promoter was studied by chromatin immuno-precipitation. Id4 was either over-expressed or silenced in prostate cancer cell lines DU145 and LNCaP respectively followed by analysis of PTEN, NKX3.1 and Sox9 expression.
Sharma, Pankaj; Knowell, Ashley; Chinaranagari, Swathi; Komaragiri, Shravan; Nagappan, Peri; Patel, Divya; Havrda, Mathew C.; and Chaudhary, Jaideep, "Id4 Deficiency Attenuates Prostate Development and Promotes PIN-like Lesions by Regulating Androgen Receptor Activity and Expression of NKX3.1 and PTEN" (2013). Open Dartmouth: Faculty Open Access Articles. 1283.