Geisel School of Medicine
Cystic fibrosis (CF) is caused by inherited mutations in the cystic fibrosis transmembrane conductance regulator gene and results in a lung environment that is highly conducive to polymicrobial infection. Over a lifetime, decreasing bacterial diversity and the presence of Pseudomonas aeruginosa in the lung are correlated with worsening lung disease. However, to date, no change in community diversity, overall microbial load or individual microbes has been shown to correlate with the onset of an acute exacerbation in CF patients. We followed 17 adult CF patients throughout the course of clinical exacerbation, treatment and recovery, using deep sequencing and quantitative PCR to characterize spontaneously expectorated sputum samples
Price KE, Hampton TH, Gifford AH, Dolben EL, Hogan DA, Morrison HG, Sogin ML, O'Toole GA. Unique microbial communities persist in individual cystic fibrosis patients throughout a clinical exacerbation. Microbiome. 2013 Nov 1;1(1):27. doi: 10.1186/2049-2618-1-27. PMID: 24451123; PMCID: PMC3971630.
Dartmouth Digital Commons Citation
Price, Katherine E.; Hampton, Thomas H.; Gifford, Alex H.; Dolben, Emily L.; Hogan, Deborah A.; Morrison, Hilary G.; Sogin, Mitchell L.; and O’Tooled, George A., "Unique Microbial Communities Persist in Individual Cystic Fibrosis Patients throughout a Clinical Exacerbation" (2013). Dartmouth Scholarship. 1297.