Biomedical Optics Express
Intraoperative 5- aminolevulinic acid induced-Protoporphyrin IX (PpIX) fluorescence guidance enables maximum safe resection of glioblastomas by providing surgeons with real-time tumor optical contrast. However, visual assessment of PpIX fluorescence is subjective and limited by the distorting effects of light attenuation and tissue autofluorescence. We have previously shown that non-invasive point measurements of absolute PpIX concentration identifies residual tumor that is otherwise non-detectable. Here, we extend this approach to wide-field quantitative fluorescence imaging by implementing spatial frequency domain imaging to recover tissue optical properties across the field-of-view in phantoms and ex vivo tissue.
Sibai, Mira; Veilleux, Israel; Elliott, Jonathan T.; Leblond, Frederic; and Wilson, Brian, "Quantitative Spatial Frequency Fluorescence Imaging in the Sub-Diffusive Domain for Image-Guided Glioma Resection" (2015). Open Dartmouth: Faculty Open Access Articles. 1339.