Title
Improved Sensitivity to Fluorescence for Cancer Detection in Wide-Field Image-Guided Neurosurgery
Document Type
Article
Publication Date
11-30-2015
Publication Title
Biomedical Optics Express
Department
Thayer School of Engineering
Abstract
In glioma surgery, Protoporphyrin IX (PpIX) fluorescence may identify residual tumor that could be resected while minimizing damage to normal brain. We demonstrate that improved sensitivity for wide-field spectroscopic fluorescence imaging is achieved with minimal disruption to the neurosurgical workflow using an electron-multiplying charge-coupled device (EMCCD) relative to a state-of-the-art CMOS system. In phantom experiments the EMCCD system can detect at least two orders-of-magnitude lower PpIX. Ex vivo tissue imaging on a rat glioma model demonstrates improved fluorescence contrast compared with neurosurgical fluorescence microscope technology, and the fluorescence detection is confirmed with measurements from a clinically-validated spectroscopic probe. Greater PpIX sensitivity in wide-field fluorescence imaging may improve the residual tumor detection during surgery with consequent impact on survival.
DOI
10.1364/BOE.6.005063
Dartmouth Digital Commons Citation
Jermyn, Michael; Gosselin, Yoann; Valdes, Pablo A.; Sibai, Mira; and Kolste, Kolbein, "Improved Sensitivity to Fluorescence for Cancer Detection in Wide-Field Image-Guided Neurosurgery" (2015). Dartmouth Scholarship. 1341.
https://digitalcommons.dartmouth.edu/facoa/1341
Included in
Bioimaging and Biomedical Optics Commons, Neurology Commons, Surgery Commons, Surgical Procedures, Operative Commons