Document Type


Publication Date


Publication Title

Molecular and Cellular Biology


Geisel School of Medicine


To understand the role of white collar-2 in theNeurospora circadian clock, we examined alleles ofwc-2 thought to encode partially functional proteins. We found that wc-2 allele ER24 contained a conservative mutation in the zinc finger. This mutation results in reduced levels of circadian rhythm-critical clock gene products, frq mRNA and FRQ protein, and in a lengthened period of the circadian clock. In addition, this mutation altered a second canonical property of the clock, temperature compensation: as temperature increased, period length decreased substantially. This temperature compensation defect correlated with a temperature-dependent increase in overall FRQ protein levels, with the relative increase being greater in wc-2(ER24) than in wild type, while overall frq mRNA levels were largely unaltered by temperature. We suggest that this temperature-dependent increase in FRQ levels partially rescues the lowered levels of FRQ resulting from the wc-2 (ER24) defect, yielding a shorter period at higher temperatures. Thus, normal activity of the essential clock component WC-2, a positive regulator offrq, is critical for establishing period length and temperature compensation in this circadian system.



Original Citation

Collett MA, Dunlap JC, Loros JJ. Circadian clock-specific roles for the light response protein WHITE COLLAR-2. Mol Cell Biol. 2001 Apr;21(8):2619-28. doi: 10.1128/MCB.21.8.2619-2628.2001. PMID: 11283242; PMCID: PMC86893.