Journal of Bacteriology
Department of Biological Sciences
Extracts of the crude bacteroid fraction of symbiotically grown Bradyrhizobium japonicum were much more active in oxidizing protoporphyrinogen to protoporphyrin than were extracts of cells grown under free-living conditions, especially when assayed in atmospheres containing only traces of oxygen. This correlates with the higher heme content of the microaerophilic nodules. Furthermore, the high level of oxidative activity in the crude bacteroid fraction was associated with an uncharacterized membrane fraction, probably of plant origin, that was separable from the bacteroids by Percoll gradient centrifugation.
Jacobs NJ, Borotz SE, Guerinot ML. Protoporphyrinogen oxidation, a step in heme synthesis in soybean root nodules and free-living rhizobia. J Bacteriol. 1989;171(1):573-576. doi:10.1128/jb.171.1.573-576.1989
Dartmouth Digital Commons Citation
Jacobs, Nicholas J.; Borotz, Susan E.; and Guerinot, Mary Lou, "Protoporphyrinogen Oxidation, a Step in Heme Synthesis in Soybean Root Nodules and Free-Living Rhizobia." (1989). Dartmouth Scholarship. 1442.