Background: We have previously demonstrated intrinsic anti-HIV activity in cervicovaginal lavage (CVL) from HIV-infected women with high CD4 counts and not on antiretroviral therapy. However, the impact of HIV disease progression on CVL innate immune responses has not been delineated.
Methods: CVL from 57 HIV-infected women not on antiretroviral therapy were collected by washing the cervicovaginal area with 10 ml of sterile normal saline. We characterized subject HIV disease progression by CD4 count strata: >500 cells/µl, 200-500 cells/µl, or <200 cells/µl of blood. To assess CVL anti-HIV activity, we incubated TZM-bl cells with HIV plus or minus CVL. Antimicrobials, cytokines, chemokines and anti-gp160 HIV IgG antibodies were measured by ELISA and Luminex.
Lahey, Timothy; Ghosh, Mimi; Fahey, John V.; Shen, Zheng; Mukura, Lucy R.; Song, Yan; Cu-Uvin, Susan; Mayer, Kenneth H.; Wright, Peter F.; Kappes, John C.; Ochsenbauer, Christina; and Wira, Charles R., "Selective Impact of HIV Disease Progression on the Innate Immune System in the Human Female Reproductive Tract" (2012). Open Dartmouth: Faculty Open Access Articles. 1549.