Document Type


Publication Date


Publication Title

Virology - Research and Treatment


Geisel School of Medicine


The immunodominant cryptic epitope SYNTGRFPPL, encoded within open reading frame 2 of the LP-BM5 retroviral gag gene, is critical for protection against retroviral-induced pathogenesis. The goal of this study was to dissect the memory response against this unique immunodominant cryptic epitope. Unlike the protective acute effector population of SYNTGRFPPL-specific CD8 T cells, long-lived SYNTGRFPPL-specific CD8 T cells lacked the ability to protect susceptible mice infected with LP-BM5 retrovirus. Compared to memory CD8 T cells against a conventional epitope with similar MHC-I specificity, primed and restimulated using similar conditions, long-lived SYNTGRFPPL-specific CD8 T cells were impaired in their ability to recall against antigen, with reduced cytolytic capabilities and cytokine production. Since similar priming and restimulation regimes were utilized to generate each effector CD8 T cell population, this study has potentially broad implications with regard to the selection criteria of potent, highly conserved cryptic epitopes for use in epitope-based vaccines.



Original Citation

Rutkowski MR, Stevens CA, Green WR. Impaired memory CD8 T cell responses against an immunodominant retroviral cryptic epitope. Virology. 2011 Apr 10;412(2):256-68. doi: 10.1016/j.virol.2010.11.013. Epub 2011 Feb 4. PMID: 21295815; PMCID: PMC3060943.