The Journal of Cell Biology
Vacuole inheritance in yeast involves the for- mation of tubular and vesicular “segregation struc- tures” which migrate into the bud and fuse there to es- tablish the daughter cell vacuole. Vacuole fusion has been reconstituted in vitro and may be used as a model for an NSF-dependent reaction of priming, docking, and fusion. We have developed biochemical and micro- scopic assays for the docking step of in vitro vacuole fusion and characterized its requirements. The vacu- oles must be primed for docking by the action of Sec17p ( a -SNAP) and Sec18p (NSF). Priming is neces- sary for both fusion partners. It produces a labile state which requires rapid docking in order to lead produc- tively to fusion. In addition to Sec17p/Sec18p, docking requires the activity of the Ras-like GTPase Ypt7p. Un- like Sec17p/Sec18p, which must act before docking, Ypt7p is directly involved in the docking process itself.
Dartmouth Digital Commons Citation
Mayer, Andreas and Wickner, William, "Docking of Yeast Vacuoles is Catalyzed by the Ras-like GTPase Ypt7p after Symmetric Priming by Sec18p (NSF)" (1997). Open Dartmouth: Peer-reviewed articles by Dartmouth faculty. 2654.