Thayer School of Engineering
Chimeric proteins boast widespread use in areas ranging from cell biology to drug delivery. Post-translational protein fusion using the bacterial transpeptidase sortase A provides an attractive alternative when traditional gene fusion fails. We describe use of this enzyme for in vitro protein ligation and report the successful fusion of 10 pairs of protein domains with preserved functionality — demonstrating the robust and facile nature of this reaction.
Levary DA, Parthasarathy R, Boder ET, Ackerman ME. Protein-protein fusion catalyzed by sortase A. PLoS One. 2011 Apr 6;6(4):e18342. doi: 10.1371/journal.pone.0018342. PMID: 21494692; PMCID: PMC3071835.
Dartmouth Digital Commons Citation
Levary, David A.; Parthasarathy, Ranganath; Boder, Eric T.; and Ackerman, Margaret E., "Protein-Protein Fusion Catalyzed by Sortase A" (2011). Dartmouth Scholarship. 3130.