Journal of Virology
Geisel School of Medicine
The core-binding factor (CBF) binds the conserved core motif in mammalian type C retrovirus enhancers. We analyzed the phosphate contacts made by CBF on the Moloney murine leukemia virus enhancer by ethylation interference assay. The phosphate contacts span 9 bp centered around the consensus core site. To examine the sequence preferences for CBF binding, we employed the technique of selected and amplified binding sequence footprinting (T. K. Blackwell and H. Weintraub, Science 250:1104-1110, 1990). The consensus binding site for CBF defined by selected and amplified binding sequence footprinting is PyGPyG GTPy.
Melnikova IN, Crute BE, Wang S, Speck NA. Sequence specificity of the core-binding factor. J Virol. 1993;67(4):2408-2411. doi:10.1128/JVI.67.4.2408-2411.1993
Dartmouth Digital Commons Citation
Melnikova, Irena N.; Crute, Barbara E.; Wang, Shuwen; and Speck, Nancy A., "Sequence Specificity of the Core-Binding Factor" (1993). Dartmouth Scholarship. 3193.