Journal of Biomedical Optics
Thayer School of Engineering
In this study, we demonstrate a method to quantify biomarker expression that uses an exogenous dual-reporter imaging approach to improve tumor signal detection. The uptake of two fluorophores, one nonspecific and one targeted to the epidermal growth factor receptor (EGFR), were imaged at 1 h in three types of xenograft tumors spanning a range of EGFR expression levels (n = 6 in each group). Using this dual-reporter imaging methodology, tumor contrast-to-noise ratio was amplified by >6 times at 1 h postinjection and >2 times at 24 h. Furthermore, by as early as 20 min postinjection, the dual-reporter imaging signal in the tumor correlated significantly with a validated marker of receptor density (P < 0.05, r = 0.93). Dual-reporter imaging can improve sensitivity and specificity over conventional fluorescence imaging in applications such as fluorescence-guided surgery and directly approximates the receptor status of the tumor, a measure that could be used to inform choices of biological therapies.
Tichauer KM, Samkoe KS, Sexton KJ, Gunn JR, Hasan T, Pogue BW. Improved tumor contrast achieved by single time point dual-reporter fluorescence imaging. J Biomed Opt. 2012 Jun;17(6):066001. doi: 10.1117/1.JBO.17.6.066001. PMID: 22734757; PMCID: PMC3381038.
Dartmouth Digital Commons Citation
Tichauer, Kenneth M.; Samkoe, Kimberley S.; Sexton, Kristian J.; Gunn, Jason R.; Tayyaba Hasan; and Pogue, Brian W., "Improved Tumor Contrast Achieved by Single Time Point Dual-Reporter Fluorescence Imaging" (2012). Dartmouth Scholarship. 3642.