Document Type


Publication Date


Publication Title

American Journal of Plant Physiology


Department of Biological Sciences


In yeast and animals, cyclins have been demonstrated to be important regulators of cell cycle progression. In recent years, a large number of A-, B-, and D-class cyclins have been isolated from a variety of plant species. One class of cyclins, the D-class cyclins, is important for progression through G1 phase of the cell cycle. In Arabidopsis, four D-class cyclins have been isolated and characterized (CYCLIN-D1;1, CYCLIN-D2;1,CYCLIN-D3;1, and CYCLIN-D4;1). In this report we describe the characterization of a fifth D-class cyclin gene,CYCLIN-D3;2 (CYCD3;2), from Arabidopsis. An enhancer trap line, line 5580, contains a T-DNA insertion inCYCD3;2. Enhancer trap line 5580 exhibits expression in young vegetative and floral primordia. In line 5580, T-DNA is inserted in the first exon of the CYCD3;2 gene; in homozygous 5580 plants CYCD3;2 RNA is not detectable. Even thoughCYCD3;2 gene function is eliminated, homozygous 5580 plants do not exhibit an obvious growth or developmental phenotype. Via in situ hybridization we demonstrate that CYCD3;2 RNA is expressed in developing vegetative and floral primordia. In addition,CYCD3;2 is also capable of rescuing a yeast strain that is deficient in G1 cyclin activity.



Original Citation

Swaminathan K, Yang Y, Grotz N, Campisi L, Jack T. An enhancer trap line associated with a D-class cyclin gene in Arabidopsis. Plant Physiol. 2000;124(4):1658-1667. doi:10.1104/pp.124.4.1658