American Journal of Plant Physiology
Department of Biological Sciences
Analysis of the ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase gene and gene products from Arabidopsis thaliana wild-type plants and the Rubisco activase-deficient mutant strain showed that the rca mutation caused GT to be changed to AT at the 5[prime]-splice junction of intron 3 in the six-intron pre-mRNA. Northern blot analysis, genomic and cDNA sequencing, and primer extension analysis indicated that the mutation causes inefficient and incomplete splicing of the pre-mRNA, resulting in the accumulation of three aberrant mRNAs. One mutant mRNA was identical with wild-type mRNA except that it included intron 3, a second mRNA comprised intron 3 and exons 4 through 7, and the third mRNA contained exons 1 through 3. The G-to-A transition is consistent with the known mechanism of mutagenesis by ethyl methanesulfonate, the mutagen used to create the Rubisco activase-deficient strain.
Orozco BM, McClung CR, Werneke JM, Ogren WL. Molecular basis of the ribulose-1,5-bisphosphate carboxylase/oxygenase activase mutation in Arabidopsis thaliana is a guanine-to-adenine transition at the 5'-splice junction of intron 3. Plant Physiol. 1993;102(1):227-232. doi:10.1104/pp.102.1.227
Dartmouth Digital Commons Citation
Orozco, Beverly M.; McClung, C Robertson; Werneke, Jeffrey M.; and Ogren, William L., "Molecular basis of the Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Activase Mutation in Arabidopsis Thaliana is a Guanine-To-Adenine Transition at the 5'-Splice Junction of Intron 3" (1993). Dartmouth Scholarship. 3683.