Journal of Biomedical Optics
Multifrequency (0 to 0.3 mm−1), multiwavelength (633, 680, 720, 800, and 820 nm) spatial frequency domain imaging (SFDI) of 5-aminolevulinic acid-induced protoporphyrin IX (PpIX) was used to recover absorption, scattering, and fluorescence properties of glioblastoma multiforme spheroids in tissue-simulating phantoms and in vivo in a mouse model. Three-dimensional tomographic reconstructions of the frequency-dependent remitted light localized the depths of the spheroids within 500 μm, and the total amount of PpIX in the reconstructed images was constant to within 30% when spheroid depth was varied. In vivo tumor-to-normal contrast was greater than ∼ 1.5 in reduced scattering coefficient for all wavelengths and was ∼ 1.3 for the tissue concentration of deoxyhemoglobin (ctHb). The study demonstrates the feasibility of SFDI for providing enhanced image guidance during surgical resection of brain tumors.
Konecky, Soren D.; Owen, Chris M.; Rice, Tyler; Valdés, Pablo A.; Owen, Chris M.; Valdes, Pablo A.; Roberts, David W.; Kolste, Kolbein; Leblond, Frederic; Paulsen, Keith D.; and Wilson, Brian C., "Spatial Frequency Domain Tomography of Protoporphyrin IX Fluorescence in Preclinical Glioma Models" (2012). Open Dartmouth: Faculty Open Access Scholarship. 3764.