Nucleic Acids Research
Geisel School of Medicine
Most methods for isolation of RNA from yeast require tedious vortexing with glass beads, and give low yields when scaled down to 10 ml cultures (1). In addition, it is frequently desirable to prepare RNA from several different yeast strains grown under a variety of growth conditions, and preparations using glass beads are impractical when dealing with multiple samples.
Schmitt ME, Brown TA, Trumpower BL. A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae. Nucleic Acids Res. 1990;18(10):3091-3092. doi:10.1093/nar/18.10.3091
Dartmouth Digital Commons Citation
Schmitt, Mark E.; Brown, Timothy A.; and Trumpower, Bernard L., "A Rapid and Simple Method for Preparation of RNA from Saccharomyces cerevisiae" (1990). Dartmouth Scholarship. 3853.