Document Type


Publication Date


Publication Title

Journal of Biomedical Optics


Thayer School of Engineering


5-Aminolevelunic acid-induced protoporphyrin IX (PpIX) fluorescence-guided resection (FGR) enables maximum safe resection of glioma by providing real-time tumor contrast. However, the subjective visual assessment and the variable intrinsic optical attenuation of tissue limit this technique to reliably delineating only high-grade tumors that display strong fluorescence. We have previously shown, using a fiber-optic probe, that quantitative assessment using noninvasive point spectroscopic measurements of the absolute PpIX concentration in tissue further improves the accuracy of FGR, extending it to surgically curable low-grade glioma. More recently, we have shown that implementing spatial frequency domain imaging with a fluorescent-light transport model enables recovery of two-dimensional images of [PpIX], alleviating the need for time-consuming point sampling of the brain surface. We present first results of this technique modified for in vivo imaging on an RG2 rat brain tumor model. Despite the moderate errors in retrieving the absorption and reduced scattering coefficients in the subdiffusive regime of 14% and 19%, respectively, the recovered [PpIX] maps agree within 10% of the point [PpIX] values measured by the fiber-optic probe, validating its potential as an extension or an alternative to point sampling during glioma resection.




needed to be re uploaded- DDC had trouble allowing revisions.

Original Citation

Sibai M, Fisher C, Veilleux I, Elliott JT, Leblond F, Roberts DW, Wilson BC. Preclinical evaluation of spatial frequency domain-enabled wide-field quantitative imaging for enhanced glioma resection. J Biomed Opt. 2017 Jul 1;22(7):76007. doi: 10.1117/1.JBO.22.7.076007. PMID: 28697235; PMCID: PMC5995142.