Biotechnology for Biofuels
Thayer School of Engineering
A previously developed mathematical model of low solids thermophilic simultaneous saccharification and fermentation (tSSF) with Avicel was unable to predict performance at high solids using a commercial cellulase preparation (Spezyme CP) and the high ethanol yield Thermoanaerobacterium saccharolyticum strain ALK2. The observed hydrolysis proceeded more slowly than predicted at solids concentrations greater than 50 g/L Avicel. Factors responsible for this inaccuracy were investigated in this study.
Podkaminer KK, Kenealy WR, Herring CD, Hogsett DA, Lynd LR. Ethanol and anaerobic conditions reversibly inhibit commercial cellulase activity in thermophilic simultaneous saccharification and fermentation (tSSF). Biotechnol Biofuels. 2012 Jun 15;5(1):43. doi: 10.1186/1754-6834-5-43. PMID: 22703989; PMCID: PMC3462704.
Dartmouth Digital Commons Citation
Podkaminer, Kara K.; Kenealy, William R.; Herring, Christopher D.; Hogsett, David A.; and Lynd, Lee R., "Ethanol and Anaerobic Conditions Reversibly Inhibit Commercial Cellulase Activity in Thermophilic Simultaneous Saccharification and Fermentation (tSSF)" (2012). Dartmouth Scholarship. 563.