BioMed Central Gastroenterology
Differentiated pancreatic acinar cells expressing endogenous levels of mutant K-Ras do not spontaneously develop pancreatic ductal adenocarcinoma (PDAC). However, we hypothesized that acinar cells would develop PDAC in the presence of Ras activity levels mimicking those of human tumor cells.We measured Ras activity in PDAC cells from mice and humans using a Raf pull-down assay. We compared the effects of acinar cell expression of mutant K-Ras at endogenous and elevated levels on Ras activity and on the development of PDAC.
Ji, Baoan; Tsou, Lilian; Wang, Huamin; Gaiser, Sebastian; Chang, David; Daniluk, Jaroslaw; Bi, Yan; Grote, Tobias; Longnecker, Daniel; and Logsdon, Craig, "Ras Activity Levels Control the Development of Pancreatic Diseases" (2009). Open Dartmouth: Faculty Open Access Articles. 591.