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BioMed Central Genetics


Geisel School of Medicine


The various steps of mRNP biogenesis (transcription, processing and export) are interconnected. It has been shown that the transcription machinery plays a pivotal role in mRNP assembly, since several mRNA export factors are recruited during transcription and physically interact with components of the transcription machinery. Although the shuttling DEAD-box protein Dbp5p is concentrated on the cytoplasmic fibrils of the NPC, previous studies demonstrated that it interacts physically and genetically with factors involved in transcription initiation. We investigated the effect of mutations affecting various components of the transcription initiation apparatus on the phenotypes of mRNA export mutant strains. Our results show that growth and mRNA export defects of dbp5 and mex67 mutant strains can be suppressed by mutation of specific transcription initiation components, but suppression was not observed for mutants acting in the very first steps of the pre-initiation complex (PIC) formation.



Original Citation

Estruch F, Hodge C, Gómez-Navarro N, Peiró-Chova L, Heath CV, Cole CN. Insights into mRNP biogenesis provided by new genetic interactions among export and transcription factors. BMC Genet. 2012 Sep 10;13:80. doi: 10.1186/1471-2156-13-80. PMID: 22963203; PMCID: PMC3506551.