Document Type

Article

Publication Date

3-21-2005

Publication Title

BioMed Central Genomics

Department

Geisel School of Medicine

Abstract

Differential gene expression specifies the highly diverse cell types that constitute the nervous system. With its sequenced genome and simple, well-defined neuroanatomy, the nematode C. elegans is a useful model system in which to correlate gene expression with neuron identity. The UNC-4 transcription factor is expressed in thirteen embryonic motor neurons where it specifies axonal morphology and synaptic function. These cells can be marked with an unc-4::GFP reporter transgene. Here we describe a powerful strategy, Micro-Array Profiling of C. elegans cells (MAPCeL), and confirm that this approach provides a comprehensive gene expression profile of unc-4::GFP motor neurons in vivo.

.

DOI

10.1186/1471-2164-6-42

Original Citation

Fox RM, Von Stetina SE, Barlow SJ, Shaffer C, Olszewski KL, Moore JH, Dupuy D, Vidal M, Miller DM 3rd. A gene expression fingerprint of C. elegans embryonic motor neurons. BMC Genomics. 2005 Mar 21;6:42. doi: 10.1186/1471-2164-6-42. PMID: 15780142; PMCID: PMC1079822.

Download

Included in

Genomics Commons

COinS