BioMed Central Genomics
Differential gene expression specifies the highly diverse cell types that constitute the nervous system. With its sequenced genome and simple, well-defined neuroanatomy, the nematode C. elegans is a useful model system in which to correlate gene expression with neuron identity. The UNC-4 transcription factor is expressed in thirteen embryonic motor neurons where it specifies axonal morphology and synaptic function. These cells can be marked with an unc-4::GFP reporter transgene. Here we describe a powerful strategy, Micro-Array Profiling of C. elegans cells (MAPCeL), and confirm that this approach provides a comprehensive gene expression profile of unc-4::GFP motor neurons in vivo.
Fox, Rebecca M.; Von Stetina, Stephen E.; Barlow, Susan J.; Shaffer, Christian; Olszewski, Kellen L.; and Moore, Jason H., "A Gene Expression Fingerprint of C. Elegans Embryonic Motor Neurons" (2005). Open Dartmouth: Faculty Open Access Articles. 607.