BioMed Central Molecular Biology
Geisel School of Medicine
Pseudo-nitzschia multiseries Hasle (Hasle) (Ps-n) is distinctive among the ecologically important marine diatoms because it produces the neurotoxin domoic acid. Although the biology of Ps-n has been investigated intensely, the characterization of the genes and biochemical pathways leading to domoic acid biosynthesis has been limited. To identify transcripts whose levels correlate with domoic acid production, we analyzed Ps-n under conditions of high and low domoic acid production by cDNA microarray technology and reverse-transcription quantitative PCR (RT-qPCR) methods. Our goals included identifying and validating robust reference genes for Ps-n RNA expression analysis under these conditions.
Boissonneault KR, Henningsen BM, Bates SS, Robertson DL, Milton S, Pelletier J, Hogan DA, Housman DE. Gene expression studies for the analysis of domoic acid production in the marine diatom Pseudo-nitzschia multiseries. BMC Mol Biol. 2013 Nov 1;14:25. doi: 10.1186/1471-2199-14-25. PMID: 24180290; PMCID: PMC3832940.
Dartmouth Digital Commons Citation
Boissonneault, Katie; Henningsen, Brooks M.; Bates, Stephen S.; Robertson, Deborah L.; Milton, Sean; Pelletier, Jerry; Hogan, Deborah A.; and Housman, David E., "Gene Expression Studies for the Analysis of Domoic Acid Production in the Marine Diatom Pseudo-Nitzschia Multiseries" (2013). Dartmouth Scholarship. 651.