BioMed Central Plant Biology
Ethylene plays critical roles in plant growth and development, including the regulation of cell expansion, senescence, and the response to biotic and abiotic stresses. Elements of the initial signal transduction pathway have been determined, but we are still defining regulatory mechanisms by which the sensitivity of plants to ethylene is modulated. We report here that members of the ARGOS gene family of Arabidopsis, previously implicated in the regulation of plant growth and biomass, function as negative feedback regulators of ethylene signaling. Expression of all four members of the ARGOS family is induced by ethylene, but this induction is blocked in ethylene-insensitive mutants. The dose dependence for ethylene induction varies among the ARGOS family members, suggesting that they could modulate responses across a range of ethylene concentrations. GFP-fusions of ARGOS and ARL localize to the endoplasmic reticulum, the same subcellular location as the ethylene receptors and other initial components of the ethylene signaling pathway. Seedlings with increased expression of ARGOS family members exhibit reduced ethylene sensitivity based on physiological and molecular responses.
Rai, Muneeza I.; Wang, Xiaomin; Thibault, Derek M.; Kim, Hyo Jung; Bombyk, Matthew M.; Binder, Brad M.; Shakeel, Samina N.; and Schaller, G. Eric, "The ARGOS Gene Family Functions in a Negative Feedback Loop to Desensitize Plants to Ethylene" (2015). Open Dartmouth: Faculty Open Access Articles. 661.