EMBO Molecular Medicine
Geisel School of Medicine
Myocardial infarction, an irreversible cardiac tissue damage, involves progressive loss of cardiomyocytes due to p53-mediated apoptosis. Oxygenation is known to promote cardiac survival through activation of NOS3 gene. We hypothesized a dual role for p53, which, depending on oxygenation, can elicit apoptotic death signals or NOS3-mediated survival signals in the infarct heart. p53 exhibited a differential DNA-binding, namely, BAX-p53RE in the infarct heart or NOS3-p53RE in the oxygenated heart, which was regulated by oxygen-induced, post- translational modification of p53. In the infarct heart, p53 was heavily acetylated at Lys118 residue, which was exclusively reversed in the oxygenated heart, apparently regulated by oxygen-dependent expression of TIP60. The inhibition of Lys118 acetylation promoted the generation of NOS3-promoting prosurvival form of p53. Thus, oxygenation switches p53-DNA interaction by regulating p53 core- domain acetylation, promoting a prosurvival transcription activity of p53. Understanding this novel oxygen-p53 survival pathway will open new avenues in cardioprotection molecular therapy.
Gogna R, Madan E, Khan M, Pati U, Kuppusamy P. p53's choice of myocardial death or survival: Oxygen protects infarct myocardium by recruiting p53 on NOS3 promoter through regulation of p53-Lys(118) acetylation. EMBO Mol Med. 2013 Nov;5(11):1662-83. doi: 10.1002/emmm.201202055. Epub 2013 Oct 1. PMID: 24096875; PMCID: PMC3840484.
Dartmouth Digital Commons Citation
Gogna, Rajan; Madan, Esha; Khan, Mahmood; Pati, Uttam; and Kuppusamy, Periannan, "P53's Choice of Myocardial Death or Survival: Oxygen Protects Infarct Myocardium by Recruiting P53 on NOS3 Promoter through Regulation of P53-Lys118 Acetylation" (2013). Dartmouth Scholarship. 799.