Whole-Genome Sequencing of Staphylococcus aureus Strain RN4220, a Key Laboratory Strain Used in Virulence Research, Identifies Mutations That Affect Not Only Virulence Factors but Also the Fitness of the Strain

Authors

Dhanalakshmi Nair, Dartmouth College
Guido Memmi, Dartmouth College
David Hernandez, University of Geneva
Jonathan Bard, New York State Center of Excellence for Bioinformatics and Life Sciences
Marie Beaume, University of GenevaFollow
Steven Gill, University of Rochester
Patrice Francois, University of Geneva
Ambrose L. Cheung, Dartmouth CollegeFollow

Document Type

Article

Publication Date

3-4-2011

Publication Title

Journal of Bacteriology

Department

Geisel School of Medicine

Abstract

Staphylococcus aureus RN4220, a cloning intermediate, is sometimes used in virulence, resistance, and metabolic studies. Using whole-genome sequencing, we showed that RN4220 differs from NCTC8325 and contains a number of genetic polymorphisms that affect both virulence and general fitness, implying a need for caution in using this strain for such studies.

DOI

10.1128/JB.00027-11

Original Citation

Nair D, Memmi G, Hernandez D, Bard J, Beaume M, Gill S, Francois P, Cheung AL. Whole-genome sequencing of Staphylococcus aureus strain RN4220, a key laboratory strain used in virulence research, identifies mutations that affect not only virulence factors but also the fitness of the strain. J Bacteriol. 2011 May;193(9):2332-5. doi: 10.1128/JB.00027-11. Epub 2011 Mar 4. PMID: 21378186; PMCID: PMC3133102.

Dartmouth Digital Commons Citation

Nair, Dhanalakshmi; Memmi, Guido; Hernandez, David; Bard, Jonathan; Beaume, Marie; Gill, Steven; Francois, Patrice; and Cheung, Ambrose L., "Whole-Genome Sequencing of Staphylococcus aureus Strain RN4220, a Key Laboratory Strain Used in Virulence Research, Identifies Mutations That Affect Not Only Virulence Factors but Also the Fitness of the Strain" (2011). Dartmouth Scholarship. 1057.
https://digitalcommons.dartmouth.edu/facoa/1057