Document Type
Article
Publication Date
2-19-2001
Publication Title
Molecular and Cellular Biology
Department
Geisel School of Medicine
Abstract
Nuclear export of ribosomes requires a subset of nucleoporins and the Ran system, but specific transport factors have not been identified. Using a large subunit reporter (Rpl25p-eGFP), we have isolated several temperature-sensitive ribosomal export (rix) mutants. One of these corresponds to the ribosomal protein Rpl10p, which interacts directly with Nmd3p, a conserved and essential protein associated with 60S subunits. We find that thermosensitive nmd3 mutants are impaired in large subunit export. Strikingly, Nmd3p shuttles between the nucleus and cytoplasm and is exported by the nuclear export receptor Xpo1p. Moreover, we show that export of 60S subunits is Xpo1p dependent. We conclude that nuclear export of 60S subunits requires the nuclear export sequence-containing nonribosomal protein Nmd3p, which directly binds to the large subunit protein Rpl10p.
DOI
10.1128/MCB.21.10.3405-3415.2001
Original Citation
Gadal O, Strauss D, Kessl J, Trumpower B, Tollervey D, Hurt E. Nuclear export of 60s ribosomal subunits depends on Xpo1p and requires a nuclear export sequence-containing factor, Nmd3p, that associates with the large subunit protein Rpl10p. Mol Cell Biol. 2001;21(10):3405-3415. doi:10.1128/MCB.21.10.3405-3415.2001
Dartmouth Digital Commons Citation
Gadal, Olivier; Strau, Daniela; Kessl, Jacques; and Trumpower, Bernard, "Nuclear Export of 60S Ribosomal Subunits Depends on Xpo1p and Requires a Nuclear Export Sequence-Containing Factor, Nmd3p, That Associates with the Large Subunit Protein Rpl10p" (2001). Dartmouth Scholarship. 1069.
https://digitalcommons.dartmouth.edu/facoa/1069
