Document Type
Article
Publication Date
11-27-2006
Publication Title
Molecular and Cellular Biology
Department
Geisel School of Medicine
Abstract
Eukaryotic genomes are packaged with histones and accessory proteins in the form of chromatin. RNA polymerases and their accessory proteins are sufficient for transcription of naked DNA, but not of chromatin, templates in vitro. In this study, we purified and identified nucleolin as a protein that allows RNA polymerase II to transcribe nucleosomal templates in vitro. As immunofluorescence confirmed that nucleolin localizes primarily to nucleoli with RNA polymerase I, we demonstrated that nucleolin allows RNA polymerase I transcription of chromatin templates in vitro. The results of chromatin immunoprecipitation experiments established that nucleolin is associated with chromatin containing rRNA genes transcribed by RNA polymerase I but not with genes transcribed by RNA polymerase II or III. Knockdown of nucleolin by RNA interference resulted in specific inhibition of RNA polymerase I transcription. We therefore propose that an important function of nucleolin is to permit RNA polymerase I to transcribe nucleolar chromatin.
DOI
10.1128/MCB.01584-06
Original Citation
Rickards B, Flint SJ, Cole MD, LeRoy G. Nucleolin is required for RNA polymerase I transcription in vivo. Mol Cell Biol. 2007 Feb;27(3):937-48. doi: 10.1128/MCB.01584-06. Epub 2006 Nov 27. PMID: 17130237; PMCID: PMC1800701.
Dartmouth Digital Commons Citation
Rickards, Brendan; Flint, S.; Cole, Michael D.; and LeRoy, Gary, "Nucleolin Is Required for RNA Polymerase I Transcription In Vivo" (2006). Dartmouth Scholarship. 1133.
https://digitalcommons.dartmouth.edu/facoa/1133
