The Growth of Simian Virus 40 (SV40) Host Range/Adenovirus Helper Function Mutants in an African Green Monkey Cell Line that Constitutively Expresses the SV40 Agnoprotein.

Authors

Terryl P. Stacy, Dartmouth College
Michele Chamberlain, Dartmouth CollegeFollow
Susan Carswell, University of Pennsylvania
Charles N. Cole, Dartmouth CollegeFollow

Document Type

Article

Publication Date

7-1990

Publication Title

Journal of Virology

Department

Geisel School of Medicine

Abstract

The simian virus 40 T-antigen carboxy-terminal mutants, dlA2459 and dlA2475, are cell line and temperature dependent for growth and plaque formation in monkey kidney cells. Although these mutants did form plaques on BSC-1 cells at 37 degrees C, they were about fivefold less efficient for plaque formation than wild-type simian virus 40. These mutants did not grow in CV-1 cells and did not synthesize agnoprotein in those cells. CV-1 cells which constitutively express the agnoprotein were permissive for mutant plaque formation. However, late mRNAs, virion proteins, and progeny virion yields did not accumulate to wild-type levels during mutant infection of the agnoprotein-producing cells.

Original Citation

Stacy TP, Chamberlain M, Carswell S, Cole CN. The growth of simian virus 40 (SV40) host range/adenovirus helper function mutants in an African green monkey cell line that constitutively expresses the SV40 agnoprotein. J Virol. 1990;64(7):3522-3526. doi:10.1128/JVI.64.7.3522-3526.1990

Dartmouth Digital Commons Citation

Stacy, Terryl P.; Chamberlain, Michele; Carswell, Susan; and Cole, Charles N., "The Growth of Simian Virus 40 (SV40) Host Range/Adenovirus Helper Function Mutants in an African Green Monkey Cell Line that Constitutively Expresses the SV40 Agnoprotein." (1990). Dartmouth Scholarship. 1177.
https://digitalcommons.dartmouth.edu/facoa/1177