Document Type
Article
Publication Date
8-1-2012
Publication Title
Biomedical Optics Express
Department
Geisel School of Medicine
Abstract
Diffuse optical remission spectra from the mammalian neocortex at visible wavelengths contain spectral features originating from the mitochondria. A new algorithm is presented, based on analytically relating the first differential of the attenuation spectrum to the first differential of the chromophore spectra, that can separate and calculate the oxidation state of cytochrome c as well as the absolute concentration and saturation of hemoglobin. The algorithm is validated in phantoms and then tested on the neocortex of the rat during an anoxic challenge. Implementation of the algorithm will provide detailed information of mitochondrial oxygenation and mitochondrial function in physiological studies of the mammalian brain.
DOI
10.1364/BOE.3.001933
Original Citation
Sakata Y, Abajian M, Ripple MO, Springett R. Measurement of the oxidation state of mitochondrial cytochrome c from the neocortex of the mammalian brain. Biomed Opt Express. 2012 Aug 1;3(8):1933-46. doi: 10.1364/BOE.3.001933. Epub 2012 Jul 25. PMID: 22876356; PMCID: PMC3409711.
Dartmouth Digital Commons Citation
Sakata, Y.; Abajian, M.; Ripple, M. O.; and Springett, R., "Measurement of the Oxidation State of Mitochondrial Cytochrome c from the Neocortex of the Mammalian Brain" (2012). Dartmouth Scholarship. 1230.
https://digitalcommons.dartmouth.edu/facoa/1230
