Absence of a Structural Basis for Intracellular Recognition and Differential Localization of Nuclear and Plasma Membrane-Associated Forms of Simian Virus 40 Large Tumor Antigen.

Authors

Donald L. Jarvis, Baylor College of Medicine
Charles N. Cole, Dartmouth CollegeFollow
Janet S. Butel, Baylor College of Medicine

Document Type

Article

Publication Date

3-1986

Publication Title

Molecular and Cellular Biology

Department

Geisel School of Medicine

Abstract

The simian virus 40 large tumor antigen (T-ag) is found in both the nuclei (nT-ag) and plasma membranes (mT-ag) of simian virus 40-infected or -transformed cells. It is not known how newly synthesized T-ag molecules are recognized, sorted, and transported to their ultimate subcellular destinations. One possibility is that these events depend upon structural differences between nT-ag and mT-ag. To test this possibility, we compared the structures of nT-ag and mT-ag from simian virus 40-infected cells. No differences between the two forms of T-ag were detected by migration in polyacrylamide gels, by Staphylococcus aureus V8 partial proteolytic mapping of methionine- or proline-containing peptides, or by two-dimensional tryptic peptide mapping of methionine-containing peptides. The carboxy-terminal, methionine-containing tryptic peptide was identified in the two-dimensional maps and was shown to be identical in nT-ag and mT-ag. Thus, a structural basis for the recognition and differential localization of T-ags could not be demonstrated. The carboxy terminus of the T-ag encoded by mutant dlA2413 is derived from the alternate open reading frame of the simian virus 40 early region, in analogy with the theoretical early gene product, T*-ag. We used this mutant to identify peptides unique to T*-ag. None of these peptides were detected in maps of mT-ag; only wild-type T-ag-specific peptides were found. These findings suggest that T*-ag does not represent the membrane-associated form of T-ag, but that mT-ag is encoded within the same reading frame used for nT-ag.

DOI

10.1128/MCB.6.3.758

Original Citation

Jarvis DL, Cole CN, Butel JS. Absence of a structural basis for intracellular recognition and differential localization of nuclear and plasma membrane-associated forms of simian virus 40 large tumor antigen. Mol Cell Biol. 1986;6(3):758-767. doi:10.1128/mcb.6.3.758

Dartmouth Digital Commons Citation

Jarvis, Donald L.; Cole, Charles N.; and Butel, Janet S., "Absence of a Structural Basis for Intracellular Recognition and Differential Localization of Nuclear and Plasma Membrane-Associated Forms of Simian Virus 40 Large Tumor Antigen." (1986). Dartmouth Scholarship. 1281.
https://digitalcommons.dartmouth.edu/facoa/1281