Microdose Fluorescence Imaging of ABY-029 on an Operating Microscope Adapted by Custom Illumination and Imaging Modules

Authors

Jonathan T. Elliott, Dartmouth CollegeFollow
Alisha V. Dsouza, Dartmouth CollegeFollow
Kayla Marra, Dartmouth College
Brian W. Pogue, Dartmouth College
David Roberts, Dartmouth College
Keith Paulsen, Dartmouth College

Document Type

Article

Publication Date

9-1-2016

Publication Title

Biomedical Optics Express

Department

Thayer School of Engineering

Additional Department

Geisel School of Medicine

Abstract

Fluorescence guided surgery has the potential to positively impact surgical oncology; current operating microscopes and stand-alone imaging systems are too insensitive or too cumbersome to maximally take advantage of new tumor-specific agents developed through the microdose pathway. To this end, a custom-built illumination and imaging module enabling picomolar-sensitive near-infrared fluorescence imaging on a commercial operating microscope is described. The limits of detection and system specifications are characterized, and in vivo efficacy of the system in detecting ABY-029 is evaluated in a rat orthotopic glioma model following microdose injections, showing the suitability of the device for microdose phase 0 clinical trials.

DOI

10.1364/BOE.7.003280

Dartmouth Digital Commons Citation

Elliott, Jonathan T.; Dsouza, Alisha V.; Marra, Kayla; Pogue, Brian W.; Roberts, David; and Paulsen, Keith, "Microdose Fluorescence Imaging of ABY-029 on an Operating Microscope Adapted by Custom Illumination and Imaging Modules" (2016). Dartmouth Scholarship. 1361.
https://digitalcommons.dartmouth.edu/facoa/1361