Document Type
Article
Publication Date
8-1982
Publication Title
Journal of Bacteriology
Department
Geisel School of Medicine
Abstract
A deletion mutant of the colicin E1-derived plasmid, pDMS6642, exhibited an approximately fourfold increase in copy number. We subsequently isolated hydroxylamine-induced mutants of that plasmid that had a further increase in copy number. Analysis of them suggests that the increased copy number of pDMS6642 is associated with transcriptional readthrough from a Tn3 transposon into the region of ColE1 containing information that influences plasmid replication. The hydroxylamine mutation in one copy number mutant appeared to increase the plasmid copy number by stimulating readthrough transcription from the Tn3 transposon into the ColE1 replication control region, whereas the other hydroxylamine mutation acts by another mechanism.
Original Citation
Schmidt L, Inselburg J. ColE1 copy number mutants. J Bacteriol. 1982;151(2):845-854. doi:10.1128/JB.151.2.845-854.1982
Dartmouth Digital Commons Citation
Schmidt, Londa and Inselburg, Joseph, "ColE1 Copy Number Mutants." (1982). Dartmouth Scholarship. 1459.
https://digitalcommons.dartmouth.edu/facoa/1459
