Document Type
Article
Publication Date
12-20-2011
Publication Title
Plant Methods
Department
Department of Biological Sciences
Abstract
Transcriptional activity of entire genes in chloroplasts is usually assayed by run-on analyses. To determine not only the overall intensity of transcription of a gene, but also the rate of transcription from a particular promoter, we created the Reverse RNase Protection Assay (RePro): in-organello run-on transcription coupled to RNase protection to define distinct transcript ends during transcription. We demonstrate successful application of RePro in plastid promoter analysis and transcript 3' end processing.
DOI
10.1186/1746-4811-7-47
Original Citation
Zubo YO, Kusnetsov VV, Börner T, Liere K. Reverse protection assay: a tool to analyze transcriptional rates from individual promoters. Plant Methods. 2011 Dec 20;7(1):47. doi: 10.1186/1746-4811-7-47. PMID: 22185205; PMCID: PMC3259058.
Dartmouth Digital Commons Citation
Zubo, Yan O.; Kusnetsov, Victor V.; Börner, Thomas; and Liere, Karsten, "Reverse Protection Assay: A Tool to Analyze Transcriptional Rates from Individual Promoters" (2011). Dartmouth Scholarship. 1543.
https://digitalcommons.dartmouth.edu/facoa/1543
