Document Type

Article

Publication Date

12-20-2011

Publication Title

Plant Methods

Department

Department of Biological Sciences

Abstract

Transcriptional activity of entire genes in chloroplasts is usually assayed by run-on analyses. To determine not only the overall intensity of transcription of a gene, but also the rate of transcription from a particular promoter, we created the Reverse RNase Protection Assay (RePro): in-organello run-on transcription coupled to RNase protection to define distinct transcript ends during transcription. We demonstrate successful application of RePro in plastid promoter analysis and transcript 3' end processing.

DOI

10.1186/1746-4811-7-47

Original Citation

Zubo YO, Kusnetsov VV, Börner T, Liere K. Reverse protection assay: a tool to analyze transcriptional rates from individual promoters. Plant Methods. 2011 Dec 20;7(1):47. doi: 10.1186/1746-4811-7-47. PMID: 22185205; PMCID: PMC3259058.

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