Transcriptional activity of entire genes in chloroplasts is usually assayed by run-on analyses. To determine not only the overall intensity of transcription of a gene, but also the rate of transcription from a particular promoter, we created the Reverse RNase Protection Assay (RePro): in-organello run-on transcription coupled to RNase protection to define distinct transcript ends during transcription. We demonstrate successful application of RePro in plastid promoter analysis and transcript 3' end processing.
Dartmouth Digital Commons Citation
Zubo, Yan O.; Kusnetsov, Victor V.; Börner, Thomas; and Liere, Karsten, "Reverse Protection Assay: A Tool to Analyze Transcriptional Rates from Individual Promoters" (2011). Open Dartmouth: Published works by Dartmouth faculty. 1543.