Transcriptional activity of entire genes in chloroplasts is usually assayed by run-on analyses. To determine not only the overall intensity of transcription of a gene, but also the rate of transcription from a particular promoter, we created the Reverse RNase Protection Assay (RePro): in-organello run-on transcription coupled to RNase protection to define distinct transcript ends during transcription. We demonstrate successful application of RePro in plastid promoter analysis and transcript 3' end processing.
Zubo, Yan O.; Kusnetsov, Victor V.; Börner, Thomas; and Liere, Karsten, "Reverse Protection Assay: A Tool to Analyze Transcriptional Rates from Individual Promoters" (2011). Open Dartmouth: Faculty Open Access Articles. 1543.