Adaptive Resistance to an Inhibitor of Chromosomal Instability in Human Cancer Cells
Document Type
Article
Publication Date
11-8-2016
Publication Title
Cell Reports
Department
Geisel School of Medicine
Abstract
Karyotype diversity is a hallmark of solid tumors that contributes to intratumor heterogeneity. This diversity is generated by persistent chromosome mis-segregation associated with chromosomal instability (CIN). CIN correlates with tumor relapse and is thought to promote drug resistance by creating a vast genomic landscape through which karyotypically unique clones survive lethal drug selection. We explore this proposition using a small molecule (UMK57) that suppresses chromosome mis-segregation in CIN cancer cells by potentiating the activity of the kinesin-13 protein MCAK. Sublethal doses of UMK57 destabilize kinetochore-microtubule (k-MT) attachments during mitosis to increase chromosome segregation fidelity. Surprisingly, chromosome mis-segregation rebounds in UMK57-treated cancer cells within a few days. This rapid relapse is driven by alterations in the Aurora B signaling pathway that hyper-stabilize k-MT attachments and is reversible following UMK57 removal. Thus, cancer cells display adaptive resistance to therapies targeting CIN through rapid and reversible changes to mitotic signaling networks.
DOI
10.1016/j.celrep.2016.10.030
Dartmouth Digital Commons Citation
Orr, Bernardo; Talje, Lama; Liu, Zhexian; and Kwok, Benjamin H., "Adaptive Resistance to an Inhibitor of Chromosomal Instability in Human Cancer Cells" (2016). Dartmouth Scholarship. 227.
https://digitalcommons.dartmouth.edu/facoa/227
