Document Type

Article

Publication Date

3-24-2004

Publication Title

Acta Crystallographica. Section D - Biological Crystallography

Department

Department of Computer Science

Abstract

Molecular replacement (MR) often plays a prominent role in determining initial phase angles for structure determination by X-ray crystallography. In this paper, an efficient quaternion-based algorithm is presented for analyzing peaks from a cross-rotation function in order to identify model orientations consistent with proper non-crystallographic symmetry (NCS) and to generate proper NCS-consistent orientations missing from the list of cross-rotation peaks. The algorithm, CRANS, analyzes the rotation differences between each pair of cross-rotation peaks to identify finite subgroups. Sets of rotation differences satisfying the subgroup axioms correspond to orientations compatible with the correct proper NCS. The CRANS algorithm was first tested using cross-rotation peaks computed from structure-factor data for three test systems and was then used to assist in the de novo structure determination of dihydrofolate reductase-thymidylate synthase (DHFR-TS) from Cryptosporidium hominis. In every case, the CRANS algorithm runs in seconds to identify orientations consistent with the observed proper NCS and to generate missing orientations not present in the cross-rotation peak list. The CRANS algorithm has application in every molecular-replacement phasing effort with proper NCS.

DOI

10.1107/S090744490400695X

Original Citation

Lilien RH, Bailey-Kellogg C, Anderson AC, Donald BR. A subgroup algorithm to identify cross-rotation peaks consistent with non-crystallographic symmetry. Acta Crystallogr D Biol Crystallogr. 2004 Jun;60(Pt 6):1057-67. doi: 10.1107/S090744490400695X. Epub 2004 May 21. PMID: 15159565.

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