Document Type
Article
Publication Date
5-1-1983
Publication Title
Journal of Clinical Investigation
Department
Geisel School of Medicine
Abstract
Acute iron loading of rats, by intraperitoneal administration of iron-dextran (500 mg Fe/kg body wt 18-20 h before killing) decreased by 30% the rate of conversion of 5-amino-[14C]levulinate ([14C]ALA) into heme as measured with a recently described procedure for liver homogenates (1981. Biochem. J. 198: 595-604). The decrease in conversion of labeled ALA into heme caused by iron loading was shown to be due to a 70-80% decrease in activity of ALA dehydrase. The decrease in activity of ALA dehydrase caused by iron loading was not associated with a decrease in hepatic concentrations of GSH, nor could it be reversed by addition of dithiothreitol, Zn2+ or chelators of Fe2+ and Fe3+. Addition of FeSO4, ferric citrate, or ferritin to homogenates of control liver had no effect of activity of ALA dehydrase. The decrease in activity of ALA dehydrase, caused by iron-dextran, was mirrored by a reciprocal increase in ALA synthase. Iron-dextran potentiated the induction of ALA synthase by allylisopropylacetamide. However, this potentiation could be dissociated from the decrease in ALA dehydrase caused by iron loading.
DOI
10.1172/JCI110866
Original Citation
Bonkowsky HL, Healey JF, Sinclair PR, Sinclair JF, Shedlofsky SI, Elder GH. Iron and the liver: acute effects of iron-loading on hepatic heme synthesis of rats. Role of decreased activity of 5-aminolevulinate dehydrase. J Clin Invest. 1983;71(5):1175-1182. doi:10.1172/jci110866
Dartmouth Digital Commons Citation
Bonkowsky, H L.; Healey, J F.; Sinclair, P R.; Sinclair, J F.; Shedlofsky, S I.; and Elder, G H., "Iron and the Liver: Acute Effects of Iron-Loading on Hepatic Heme Synthesis of Rats. Role of Decreased Activity of 5-Aminolevulinate Dehydrase." (1983). Dartmouth Scholarship. 3614.
https://digitalcommons.dartmouth.edu/facoa/3614
