Document Type

Article

Publication Date

7-1996

Publication Title

American Journal of Plant Physiology

Department

Department of Biological Sciences

Abstract

To identify possible iron sources for bacteroids in planta, soybean (Glycine max L. Merr.) symbiosomes (consisting of the bacteroid-containing peribacteroid space enclosed by the peribacteroid membrane [PBM]) and bacteroids were assayed for the ability to transport iron supplied as various ferric [Fe(III)]-chelates. Iron presented as a number of Fe(III)-chelates was transported at much higher rates across the PBM than across the bacteroid membranes, suggesting the presence of an iron storage pool in the peribacteroid space. Pulse-chase experiments confirmed the presence of such an iron storage pool. Because the PBM is derived from the plant plasma membrane, we reasoned that it may possess a ferric-chelate reductase activity similar to that present in plant plasma membrane. We detected ferric-chelate reductase activity associated with the PBM and suggest that reduction of Fe(III) to ferrous [Fe(II)] plays a role in the movement of iron into soybean symbiosomes.

DOI

10.1104/pp.111.3.893

Original Citation

LeVier K, Day DA, Guerinot ML. Iron Uptake by Symbiosomes from Soybean Root Nodules. Plant Physiol. 1996;111(3):893-900. doi:10.1104/pp.111.3.893

Download
COinS