Document Type
Article
Publication Date
7-12-2012
Publication Title
Genome Biology and Evolution
Department
Department of Biological Sciences
Abstract
Here we describe the genome of Mesotoga prima MesG1.Ag4.2, the first genome of a mesophilic Thermotogales bacterium. Mesotoga prima was isolated from a polychlorinated biphenyl (PCB)-dechlorinating enrichment culture from Baltimore Harbor sediments. Its 2.97 Mb genome is considerably larger than any previously sequenced Thermotogales genomes, which range between 1.86 and 2.30 Mb. This larger size is due to both higher numbers of protein-coding genes and larger intergenic regions. In particular, the M. prima genome contains more genes for proteins involved in regulatory functions, for instance those involved in regulation of transcription. Together with its closest relative, Kosmotoga olearia, it also encodes different types of proteins involved in environmental and cell–cell interactions as compared with other Thermotogales bacteria. Amino acid composition analysis of M. prima proteins implies that this lineage has inhabited low-temperature environments for a long time. A large fraction of the M. prima genome has been acquired by lateral gene transfer (LGT): a DarkHorse analysis suggests that 766 (32%) of predicted protein-coding genes have been involved in LGT after Mesotoga diverged from the other Thermotogales lineages. A notable example of a lineage-specific LGT event is a reductive dehalogenase gene—a key enzyme in dehalorespiration, indicating M. prima may have a more active role in PCB dechlorination than was previously assumed.
DOI
10.1093/gbe/evs059
Original Citation
Zhaxybayeva O, Swithers KS, Foght J, Green AG, Bruce D, Detter C, Han S, Teshima H, Han J, Woyke T, Pitluck S, Nolan M, Ivanova N, Pati A, Land ML, Dlutek M, Doolittle WF, Noll KM, Nesbø CL. Genome sequence of the mesophilic Thermotogales bacterium Mesotoga prima MesG1.Ag.4.2 reveals the largest Thermotogales genome to date. Genome Biol Evol. 2012;4(8):700-8. doi: 10.1093/gbe/evs059. Epub 2012 Jul 12. PMID: 22798451; PMCID: PMC3516359.
Dartmouth Digital Commons Citation
Zhaxybayeva, Olga; Swithers, Kristen S.; Foght, Julia; Green, Anna G.; Bruce, David; Detter, Chris; Han, Shunsheng; Teshima, Hazuki; Han, James; Woyke, Tanja; Pitluck, Sam; Nolan, Matt; Ivanova, Natalia; Pati, Amrita; Land, Miriam L.; Dlutek, Marlena; Doolittle, W Ford; Noll, Kenneth M.; and Nesbo, Camilla L., "Genome Sequence of the Mesophilic Thermotogales Bacterium Mesotoga prima MesG1.Ag.4.2 Reveals the Largest Thermotogales Genome to Date" (2012). Dartmouth Scholarship. 3795.
https://digitalcommons.dartmouth.edu/facoa/3795
