Document Type

Article

Publication Date

2-18-2009

Publication Title

Molecular Biology of the Cell

Department

Department of Biological Sciences

Abstract

Septins are conserved, GTP-binding proteins that assemble into higher order structures, including filaments and rings with varied cellular functions. Using four-dimensional quantitative fluorescence microscopy of Ashbya gossypii fungal cells, we show that septins can assemble into morphologically distinct classes of rings that vary in dimensions, intensities, and positions within a single cell. Notably, these different classes coexist and persist for extended times, similar in appearance and behavior to septins in mammalian neurons and cultured cells. We demonstrate that new septin proteins can add through time to assembled rings, indicating that septins may continue to polymerize during ring maturation. Different classes of rings do not arise from the presence or absence of specific septin subunits and ring maintenance does not require the actin and microtubule cytoskeletons. Instead, morphological and behavioral differences in the rings require the Elm1p and Gin4p kinases. This work demonstrates that distinct higher order septin structures form within one cell because of the action of specific kinases.

DOI

10.1091/mbc.E08-12-1169

Original Citation

DeMay BS, Meseroll RA, Occhipinti P, Gladfelter AS. Regulation of distinct septin rings in a single cell by Elm1p and Gin4p kinases. Mol Biol Cell. 2009 Apr;20(8):2311-26. doi: 10.1091/mbc.e08-12-1169. Epub 2009 Feb 18. PMID: 19225152; PMCID: PMC2669037.

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