Document Type

Article

Publication Date

9-13-2018

Publication Title

Proceedings of SPIE-- The International Society for Optical Engineering

Department

Thayer School of Engineering

Abstract

Dynamic fluorescence imaging approaches can be used to estimate the concentration of cell surface receptorsin vivo. Kinetic models are used to generate the final estimation by taking the targeted imaging agent concentration as a function of time. However, tissue absorption and scattering properties cause the final readout signal to be on a different scale than the real fluorescent agent concentration. In paired-agent imaging approaches, simultaneous injection of a suitable control imaging agent with a targeted one can account for non-specific uptake and retention of the targeted agent. Additionally, the signal from the control agent can be a normalizing factor to correct for tissue optical property differences. In this study, the kinetic model used for paired-agent imaging analysis (i.e., simplified reference tissue model) is modified and tested in simulation and experimental data in a way that accounts for the scaling correction within the kinetic model fit to the data to ultimately extract an estimate of the targeted biomarker concentration.

DOI

10.1117/12.2290631

Comments

Copyright 2018 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

Original Citation

Sadeghipour, Negar et al. “Quantifying cancer cell receptors with paired-agent fluorescent imaging: a novel method to account for tissue optical property effects.” Proceedings of SPIE--the International Society for Optical Engineering vol. 10497 (2018): 1049723. doi:10.1117/12.2290631

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