Trinity Transcript Assembly for the Titan Arum

Authors

Alveena Zulfiqar, Dartmouth CollegeFollow
Beenish J. Azhar, Dartmouth CollegeFollow
Samina N. Shakeel, Dartmouth CollegeFollow
William Thives Santos, University of MissouriFollow
Theresa D. Barry, Dartmouth CollegeFollow
Dana Ozimek, Dartmouth CollegeFollow
Kim DeLong, Dartmouth CollegeFollow
Ruthie Angelovici, University of MissouriFollow
Kathleen M. Greenham, University of Minnesota - Twin CitiesFollow
Craig A. Schenck, University of MissouriFollow
G. Eric Schaller, Dartmouth CollegeFollow

Author ORCID Identifier

https://orcid.org/0000-0003-4032-2437

Document Type

Article

Publication Date

2024

Department

Department of Biological Sciences

Abstract

Transcriptome analysis of the titan arum was performed using floral and leaf tissues harvested from “Morphy” and “Morphy Junior,” two titan arums housed in the greenhouse of the Life Sciences Center at Dartmouth College. De novo transcript assembly for the reads obtained by RNA-seq was performed with Trinity-v2.6.6, using the default settings of >95% identity and a minimum assembled contig length of 200 nucleotides, resulting in the assembly of 915,085 transcripts that clustered as 536,644 genes.

See the following publication for details:

Zulfiqar, A., Azhar, B.J., Shakeel, S.N., Thives Santos, W., Barry, T.D., Ozimek, D., DeLong, K., Angelovici, R., Greenham, K.M., Schenck, C.A., and Schaller, G.E. (2024) Molecular basis for thermogenesis and volatile production in the titan arum. PNAS Nexus. 3:pgae492. doi: 10.1093/pnasnexus/pgae492

Dartmouth Digital Commons Citation

Zulfiqar, Alveena; Azhar, Beenish J.; Shakeel, Samina N.; Thives Santos, William; Barry, Theresa D.; Ozimek, Dana; DeLong, Kim; Angelovici, Ruthie; Greenham, Kathleen M.; Schenck, Craig A.; and Schaller, G. Eric, "Trinity Transcript Assembly for the Titan Arum" (2024). Dartmouth Scholarship. 4338.
https://digitalcommons.dartmouth.edu/facoa/4338