The Exometabolome of Clostridium Thermocellum Reveals Overflow Metabolism at High Cellulose Loading

Authors

Evert K. Holwerda, Dartmouth College
Philip G. Thorne, Mascoma Corporation
Daniel G. Olson, Dartmouth College
Daniel Amador-Noguez, University of Wisconsin-Madison
Nancy L. Engle, Oak Ridge National Laboratory
Timothy J. Tschaplinski, Oak Ridge National Laboratory
Johannes P. van Dijken, Delft University of Technology
Lee R. Lynd, Dartmouth College

Document Type

Article

Publication Date

10-21-2014

Publication Title

Biotechnology for Biofuels

Department

Thayer School of Engineering

Abstract

BackgroundClostridium thermocellum is a model thermophilic organism for the production of biofuels from lignocellulosic substrates. The majority of publications studying the physiology of this organism use substrate concentrations of ≤10 g/L. However, industrially relevant concentrations of substrate start at 100 g/L carbohydrate, which corresponds to approximately 150 g/L solids. To gain insight into the physiology of fermentation of high substrate concentrations, we studied the growth on, and utilization of high concentrations of crystalline cellulose varying from 50 to 100 g/L by C. thermocellum. .

DOI

10.1186/s13068-014-0155-1

Dartmouth Digital Commons Citation

Holwerda, Evert K.; Thorne, Philip G.; Olson, Daniel G.; Amador-Noguez, Daniel; Engle, Nancy L.; Tschaplinski, Timothy J.; van Dijken, Johannes P.; and Lynd, Lee R., "The Exometabolome of Clostridium Thermocellum Reveals Overflow Metabolism at High Cellulose Loading" (2014). Dartmouth Scholarship. 561.
https://digitalcommons.dartmouth.edu/facoa/561